2 edition of effect of suspending media on the freeze-drying and stability of an enterovirus. found in the catalog.
effect of suspending media on the freeze-drying and stability of an enterovirus.
Written in English
Thesis (Ph. D.)--The Queen"s University of Belfast, 1976.
|The Physical Object|
Nevertheless, considerable differences in the effects of stabilizers and freezing rates between structurally different viruses were detected. Although several authors have compared the stability of viruses of the same family in the lyophilization process under identical conditions (Berge et al. ; Tannock et al. Recombinant Factor VIII (rFVIII) and α‐amylase were used as model proteins to examine the effect of freeze‐drying process conditions on the long‐term stability of these proteins as freeze‐dried solids. The same sucrose/glycine formulation was used for all treatments.
processes Article Effect of the Freeze-Drying Process on the Physicochemical and Microbiological Properties of Mexican Keﬁr Grains Alicia Águeda Conde-Islas 1, Maribel Jiménez-Fernández 2, Denis Cantú-Lozano 1, Galo Rafael Urrea-García 1 and Guadalupe Luna-Solano 1,* 1 Instituto Tecnológico de Orizaba, División de Estudios de Posgrado e Investigación, Oriente 9 No. of freeze-drying when either adonitol or glutamate was present in the suspending medium. However, the former additive exerted the strongest protective effect on the via-bility oflactic acid bacteria, and a higher residual moisture content was obtained. After min ofdrying, a remarkable decrease in viable counts was found; this reduction was.
guide to the freeze drying process. The information presented is generic in nature and is the result of research and experience by Labconco personnel and users of freeze drying equipment. It is our intention to provide a non-biased review of preparation techniques and freeze drying methods. The purpose of . The complex nanoliposomes encapsulating both a hydrophilic drug vitamin C (vit C) and hydrophobic drug medium-chain fatty acids (MCFAs) was prepared by combining double emulsion method with dynamic high pressure microfluidization. The complex nanoliposomes was further freeze-dried under −86 °C for 48 h with sucrose at the sucrose/lipids ratio of (w/w) in order to enhance its stability.
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Effect of suspending media on freeze-drying and preservation of vaccinia virus. The protective effect of various suspending media was investigated both in the course of the freeze-drying and in the period of subsequent storage of the dried product at different temperatures, including ° C.
Greiff D, Rightsel WA. Stability of Cited by: 8. The Effect of Suspending Media on the Freeze-Drying and Stability of an Enterovirus.
Author: McCafferly, D. ISNI: Awarding Body: Queen's University Belfast Current Institution: Queen's University Belfast Date of Award. Effect of suspending media on freeze-drying and preservation of vaccinia virus. Suzuki M. Unpurified and purified smallpox vaccines were prepared from calf dermal pulp, or chorioallantoic membrane (CAM) of hen eggs infected with vaccinia virus, and freeze-dried.
The protective effect of various suspending media was investigated both in the Cited by: 8. To circumvent complicating situations and to assist in the search for causes of death resulting solely from freeze- drying, we restricted our selection of ma- te,'ials and procedures as follows: we pre- pared E.
coli for freezing in salt-free suspending media, freeze-dried them to completion at C, excluded oxygen, and rehydrated, (a) with Cited by: Suspending media containing glutamate, either alone or in combination with sucrose and either dextran or phosphates, afforded the greatest degree of protection during the freeze-drying process and.
This research compared the effects of vacuum freeze drying (VFD) and conventional freeze drying (CFD) processes on the stability of fish oil–loaded nanocapsules (NCs). The effects of freezing method, freeze drying process, and the use of protective agents on the viability of the biocontrol yeast Candida sake were studied.
Freezing at −20°C was the best method to preserve the viability of C. sake cells after freeze drying using 10% skim milk as a protectant (% survival). Liquid nitrogen freezing caused the highest level of damage to the cells with.
Effect of freeze-drying, cryoprotectants and storage conditions on the stability of secondary structure of insulin-loaded solid lipid nanoparticles Author links open overlay panel Sandra Soares a Pedro Fonte a b Ana Costa a José Andrade a Vítor Seabra a Domingos Ferreira c Salette Reis b Bruno Sarmento a d.
Suzuki, M. Effect of suspending media on freeze-drying and preservation of vaccinia it appears that the protein must be retained in its native-like state during freeze–drying to assure. (). Effect of Freeze-Drying Process Conditions on the Stability of Nanoparticles.
Drying Technology: Vol. 22, No.pp. The effect of additives on water activities of the cultures following freeze-drying (24 h at 30) and water content of the powders stabilized at a= Additive a^ Following freeze-drying Wateriaw = content (%) in powder Control Gelatin Maltodextrins Xanthan gum ' ^ (US3 ' '' s'47ab For a given column, values.
J Pharm Pharmacol. Dec;29 SupplP. Effect of suspending media on the freeze-drying and subsequent storage of ECBO virus serotype VG(5) 27 [proceedings]. Therefore, cryoprotectants are used to stabilize liposomal vesicles during freeze-drying process.
Objective: In the present study, we developed AmB liposomal suspension and lyophilized liposomes loaded with AmB, evaluated the effect of different cryoprotectants on the.
The objective of this work was to investigate the effect of cake collapse during freeze-drying on the stability of protein lyophilizates containing a monoclonal IgG(1)-antibody or a second.
Survival immediately after freeze-drying varied with the drying interval and composition of the suspending medium and was highest (70–%) in media containing % added glucose. Survival during storage in freeze-dried suspensions in serum containing % added glucose was inversely related to storage time and temperature; after 1 year at Our objective was to investigate the effect of performing primary drying at product temperatures below and above Tg′ (glass transition temperature of the freeze-concentrated phase) on the long-term stability of lyophilized proteins.
Two protective media differing in the nature of the bulking agent used (amorphous or crystalline) were selected. FREEZE-DRYING PROCESS: Freeze-drying process also affects the stability of product & there are various substances used for the process of freeze-drying which also leads to either increase or decrease in the stability.
EX: Freeze drying was found to have destructive effect on the ordered structure of starch & this effect varied with respect. Neither Pprim nor ΔT sec had a clear effect on IgG in vitro binding activity after freeze-drying when measured with ELISA (p > ), but generally, freeze-drying did seem to cause a small (freeze-drying when measured with AF4, but neither Pprim nor ΔT sec had a.
The purpose of this study is to investigate the effects of moisture content on the storage stability of freeze-dried lipoplex formulations.
DC-Cholesterol: DOPE (dioleoyl phosphatidylethanolamine) /plasmid DNA lipoplexes were prepared at a 3-to-2 DC-Cholesterol + to DNA − molar ratio and lyophilized prior to storing at room temperature, 40 °C, and 60 °C for three months. Composition of suspension media for (freeze)-drying. 8 Nutrient Broth (Difco ) 0,8 g myo-Inositol 1 g Distilled water ml 1 myo-inositol may be replaced by other protective compounds 9 Skim Milk, spray dried (Difco ) g Distilled water ml Ampoules are filled with ml skim milk and sterilized at °C for 13 min.
Freeze-drying is a method of removing water by sublimation of ice crystals from frozen material. Suitable parameters of process application allow us to obtain best quality products compared to.The aim of the present study was to evaluate the effect of freeze drying on thermo-responsive property of the hydrogel and structural stability of the loaded protein.
The freeze-dried EPO-loaded hydrogel were characterized using various methods.Design of freeze-drying processes is often approached with a “trial and error” experimental plan or, worse yet, the protocol used in the first laboratory run is adopted without further attempts at optimization.
Consequently, commercial freeze-drying processes are often neither robust nor efficient. It is our thesis that design of an “optimized” freeze-drying process is not particularly.